Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes.
نویسندگان
چکیده
We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 39 شماره
صفحات -
تاریخ انتشار 2015